A prolific scorer with 322 regular season goals to his name, Stamkos missed almost 11 months recovering from a torn lateral meniscus in his right knee.
Stamkos entered with five assists in three games this season.
Slater Koekkoek got his first two National Hockey League goals, and Victor Hedman and Nikita Kucherov also scored for the Lightning. Andrei Vasilevskiy made 36 saves.
Jake Guentzel and Bryan Rust each had a goal and an assist for the Penguins (2-2-1). Antti Niemi, who allowed four goals on 13 shots over 9:16 against Chicago on October 5 in his Penguins debut, stopped 29 shots.
Penguin disaster as just two chicks live
Rod Downie, head of polar programmes at conservation group WWF, said: 'These are one of the most incredible animals on our planet. As a result the chicks starved.
First Period_1, Tampa Bay, Koekkoek 1 (Stralman, Kunitz), 6:07. 5, Pittsburgh, Sheary 3 (Ruhwedel, Niemi), 8:58.
Hunwick got Pittsburgh within 5-4 at 10:01 of the third.
Hedman put the Lightning ahead 2-1 with a power-play goal that beat the buzzer at the end of the first period.
Air quality puts Cal football's Friday matchup under haze of questions
A sustained Air Quality Index of 200 or more is the threshold for delaying or canceling a competition, and the Berkeley area was at only 155 on Thursday afternoon.
Kris Letang assisted on Guentzel's goal and joined Paul Coffey (332) as the only Pittsburgh defenseman to reach 300 assists.
Power-play opportunities_Pittsburgh 1 of 5; Tampa Bay 2 of 5.
Penguins: Return home to play Florida on Saturday night.
When is the deadline to use the old pound coin?
That means people across Kent have an excuse to go out and spend their old coins, if they have any left. Nearly a third of the 500m still in circulation are locked inside children's money boxes.