The incident occurred at the Paletta International Corporation building, a meat processing plant founded in 1967 located near the busy intersection of Appleby and Mainway.
The blaze at 4480 Paletta Ct. - that's near Appleby Line and South Service Rd. - broke out around 4:10 p.m. on December 6.
Police have closed a portion of Appleby Line beside the scene of the fire, as well as the Toronto-bound access to the QEW.
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Burlington firefighters had help from their counterparts in Oakville and Hamilton.
"I honestly think we'll be here most of the rest of the day, one way or another", Burlington fire Chief David Lazenby said Thursday.
A section of the roof and the top two floors on the east side of the large building have collapsed.
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"We ask that residents travel safely, as smoke is thick near the fire location and traffic is heavy", a statement released by Burlington Fire said.
The cause and damage have yet to be determined.
No injuries had been reported to employees of the building or emergency personnel as of 7 p.m. but the Office of the Fire Marshal had been called for a fire likely to involve significant dollar damage.
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