Also named to the roster is Jonathan's older brother and Galaxy teammate Giovani who is seeking his third appearance in a World Cup.
Rafa Marquez has been included in Mexico's preliminary squad for this summer's World Cup in Russian Federation, putting him on course to take part in the tournament for a fifth time.
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Here are the defenders in contention for spots on Mexico's World Cup team.
Appearing in a fifth World Cup would be a fitting farewell for the 39-year-old Marquez, who had already announced his plans to retire from the sport following the tournament in Russian Federation. Mexico will start training camp Monday ahead of pre-World Cup exhibition games it will play in the coming weeks against Wales and Scotland.
There was little else in the way of major surprises from Osorio, although Chivas midfielder Rodolfo Pizarro was left out and there was no place for fellow midfielder Jose Juan "Gallito" Vazquez, who has been playing well at Santos Laguna.
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Mexico legend Rafa Marquez is among Mexico's midfielders.
"I am not a medical specialist, but if the World Cup began tomorrow those five could not be in it", said Osorio, adding that Erick Gutierrez could fill in for Guardado if needed.
Here are the three goalkeepers on Mexico's preliminary squad.
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Mexico kick off their campaign against champions Germany on June 17 before facing South Korea and Sweden in Group F.